All lanes : Anti-ITGA8 Antibody (C-term) at 1:2000 dilutionLane 1: Raji whole cell lysateLane 2: 293 whole cell lysateLane 3: LNCaP whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 117 kDaBlocking/Dilution buffer: 5% NFDM/TBST.