Ordering Details
Cat Number:	AP11206C
Size:	400uL
Price:	£304.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

LIN28A [Centre] [RB14827], Rabbit Polyclonal Antibody

Partner: Abcepta, Inc.

Applications:	IF, FC, IHC-P, WB
Species Reactivity:	Human, Mouse
Isotype:	Rabbit Ig
Purification:	Purified Rabbit Polyclonal Antibody
Description:	Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
Further Information:	This LIN28A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-138 amino acids from the Central region of human LIN28A.
Synonym:	Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1, LIN28A, CSDD1, LIN28, ZCCHC1
Ascension Number:	Q9H9Z2
NCBI EntrezGene:	79727
Shipping Conditions:	Ship on cold packs
Storage:	Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:	Research use only

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Fluorescent confocal image of SY5Y cells stained with AP11206c LIN28A (Center) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AP11206c LIN28A (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor� 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor� 555 (red) conjugated Phalloidin (5.25 ?M, 25 min). Lin28a immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.