All lanes : Anti-ENASE Antibody (Center) at 1:2000 dilutionLane 1: HL-60 whole cell lysateLane 2: human brain tissue lysateLane 3: mouse thymus tissue lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.