Ordering Details
Cat Number:	AP1047a-ev
Size:	80uL
Price:	£117.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

His Tag [RB19955], Rabbit Polyclonal Antibody

Partner: Abcepta, Inc.

Applications:	WB
Isotype:	Rabbit IgG
Purification:	Purified Rabbit Polyclonal Antibody
Description:	Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Further Information:	Poly-HIS peptide were used to produced this antibody.
Shipping Conditions:	Ship on cold packs
Storage:	Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:	Research use only

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Western blot analysis of His Tag Antibody (Cat. #AP1047a) in his-tag TBB1 protein (35ug/lane). His-tag TBB1 (arrow) was detected using the purified Pab.

Western blot analysis of His Tag Antibody (Cat. #AP1047a) in his-tag TBB1 protein (35ug/lane). His-tag TBB1 (arrow) was detected using the purified Pab.

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