Ordering Details
Cat Number:	AP1013a
Size:	200uL
Price:	£304.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

FLAG tag [DYKDDDDK] [RB0493], Rabbit Polyclonal Antibody

Partner: Abcepta, Inc.

Applications:	WB
Species Reactivity:	Human
Isotype:	Rabbit IgG
Purification:	Purified Rabbit Polyclonal Antibody
Description:	Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1). Abgent's DYKDDDDK antibody binds to the same epitope recognized by Sigma's Anti-FLAG antibodies. (FLAG is a registered trademark of Sigma-Aldrich Co., which is not affiliated with Abgent).
Further Information:	KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
Shipping Conditions:	Ship on cold packs
Storage:	Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:	Research use only

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All lanes : Anti-FLAG tag antibody (DYKDDDDK) at 1:2000 dilutionLane 1: 12-tag recombinant protein Lysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Observed band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.