Western blot using Affinity Purified anti-SLIT-3 antibody shows detection of a predominant band at ~145 kDa corresponding to SLIT-3 (arrowhead) in a bovine thyroid whole cell lysate using the 800 nm channel (green). ~ 35ug of lysate was separated on a 4-8% Tricine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:800. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye (TM)800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature. Molecular weight markers were used for size comparison using the 700 nm channel (not shown). IRDye (TM)800 fluorescence image was captured using the Odyssey (R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results
