Western Blot analysis AP02022SU VEGF antibody specificity. Differenet recombinant Human (rh) and Recombinant Bovine (rb) VEGF proteins were separated by SDS-PAGE and immunoblotted with AP02022SU (1/10,000). Lane 1: rhVEGF165 (200ng). Lane 2: rhVEGF189 (cell lysate). Lane 3: rbVEGF164 (50ng). Lane 4: rbVEGF164 (100ng). Lane 5: rbVEGF164 (100ng) with AP02022SU preadsorbed with 15 mg/ml of rhVEGF165. Lane M: Rainbow Marker. Berisha B et al. (2000) Biol. Reprod 63 (4): 1106-14.