Immunohistochemistry of FGF-2 staining in Paraffin sections of Bovine follicule tissue. Endogenous peroxidase activity was blocked by incubating the section in 1% H2O2 in Methanol for 30 minutes. The section was incubated with AP02009SU (1/1200) and detected using Biotinylated secondary antibody with ABC Kit. DAB was used as the Chromogen. (A) AP02009SU stains the larger vessels in the theca externa, but not lymphatic vessels (arrow). Scale bar = 100 µm. Berisha B et al (2000) J Endocrinol167 (3):371-82.