Applications: | ELISA, WB, FACS, IHC |
Species Reactivity: | Human |
Isotype: | Mouse IgG2a kappa |
Immunogen: | Recombinant human Nanog (1-154aa) purified from E. coli |
Purification: | By protein-G affinity chromatography |
Conjugation: | Unconjugated |
Description: | Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53. |
Further Information: | The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500~1:1,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:500 and Immunohistochemistry is 1:50. |
Synonym: | Nanog homeobox,FLJ12581, FLJ40451 |
Ascension Number: | Q9H9S0 |
NCBI EntrezGene: | NP_079141 |
Shipping Conditions: | Ship on cold packs |
Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Usage: | Research use only |