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All lanes : Anti-FKBP1A at 1:4000 dilutionLane 1: Jurkat whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: Mouse brain whole tissue lysateLane 4: Rat brain whole tissue lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 12 kDaBlocking/Dilution buffer: 5% NFDM/TBST.