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All lanes : Anti-CCR7 Antibody (N-term) at 1:4000 dilutionLane 1: K562 whole cell lysateLane 2: Daudi whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.