All lanes : Anti-ADK Antibody (N-term) at 1:4000 dilutionLane 1: HepG2 whole cell lysateLane 2: HL-60 whole cell lysateLane 3: Human liver lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDaBlocking/Dilution buffer: 5% NFDM/TBST.