All lanes : Anti-PGK1 Antibody at 1:8000 dilutionLane 1: 293 whole cell lysateLane 2: A431 whole cell lysateLane 3: mouse brain lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDaBlocking/Dilution buffer: 5% NFDM/TBST.