All lanes : Anti-PCNA Antibody at 1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: A431 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.