Ordering Details
Cat Number:	AM8545b-ev
Size:	50uL
Price:	£117.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

PCNA [1655CT506.10.26], Mouse Monoclonal Antibody

Partner: Abcepta, Inc.

Applications:	WB, FC, IHC-P
Species Reactivity:	Human
Isotype:	IgG1,k
Purification:	Purified Mouse Monoclonal Antibody
Description:	Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Further Information:	This PCNA antibody is generated from a mouse immunized with a recombinant protein of human PCNA.
Synonym:	Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Ascension Number:	P12004
NCBI EntrezGene:	5111
Shipping Conditions:	Ship on cold packs
Storage:	Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:	Research use only

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All lanes : Anti-PCNA Antibody at 1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: A431 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.