Ordering Details
Cat Number:	AM8514b
Size:	200uL
Price:	£304.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

MCM3 [1593CT377.41.73], Mouse Monoclonal Antibody

Partner: Abcepta, Inc.

Applications:	WB, FC
Species Reactivity:	Human, Mouse, Rat
Isotype:	IgG1,k
Purification:	Purified Mouse Monoclonal Antibody
Description:	Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Further Information:	This MCM3 antibody is generated from a mouse immunized with a recombinant protein of human MCM3.
Synonym:	DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102, MCM3
Ascension Number:	P25205
NCBI EntrezGene:	4172
Shipping Conditions:	Ship on cold packs
Storage:	Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:	Research use only

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All lanes : Anti-MCM3 Antibody at 1:4000 dilutionLane 1: CHO whole cell lysateLane 2: NIH/3T3 whole cell lysateLane 3: Hela whole cell lysateLane 4: HepG2 whole cell lysateLane 5: C2C12 whole cell lysateLane 6: PC-12 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 91 kDaBlocking/Dilution buffer: 5% NFDM/TBST.