All lanes : Anti-MPIP3 Antibody at 1:1000-1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: K562 whole cell lysateLane 3: MCF-7 whole cell lysateLane 4: Raji whole cell lysateLysates/proteins at 20 ?g per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDaBlocking/Dilution buffer: 5% NFDM/TBST.