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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line) cells labeling DPYL5 with AM8473b at 1/25 dilution, followed by Dylight� 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on SH-SY5Y cell line. The nuclear counter stain is DAPI (blue).