Ordering Details
Cat Number:	AM8471b
Size:	400uL
Price:	£304.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

IF4E [1504CT774.14.55], Mouse Monoclonal Antibody

Partner: Abcepta, Inc.

Applications:	WB, IF
Species Reactivity:	Human, Mouse, Rat
Isotype:	IgG1,?
Purification:	Purified Mouse Monoclonal Antibody
Description:	Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Further Information:	This IF4E antibody is generated from a mouse immunized with a recombinant protein of human IF4E.
Synonym:	Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F
Ascension Number:	P06730
NCBI EntrezGene:	1977
Shipping Conditions:	Ship on cold packs
Storage:	Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:	Research use only

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling IF4E with AM8471b at 1/25 dilution, followed by Dylight� 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight� 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).