Ordering Details
Cat Number:	AM8442b-ev
Size:	80uL
Price:	£117.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

RPTOR [1411CT316.2.151.34], Mouse Monoclonal Antibody

Partner: Abcepta, Inc.

Applications:	IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Isotype:	IgG1,k
Purification:	Purified Mouse Monoclonal Antibody
Description:	Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.
Further Information:	This RPTOR antibody is generated from a mouse immunized with recombinant protein.
Synonym:	Regulatory-associated protein of mTOR, Raptor, p150 target of rapamycin (TOR)-scaffold protein, RPTOR, KIAA1303, RAPTOR
Ascension Number:	Q8N122
NCBI EntrezGene:	57521
Shipping Conditions:	Ship on cold packs
Storage:	Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:	Research use only

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Western blot analysis of lysates from Hela, MCF-7, mouse C2C12, mouse NIH/3T3, rat C6 cell line (from left to right), using RPTOR Antibody(Cat. #AM8442b). AM8442b was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20?g per lane.