The recombinant protein (50ng) and cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EDAR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conj ugated to HRP and an ECL detection system. Lane 1 : Recombinant protein Lane 2 : A549 cell lysate Lane 3 : Ramos cell lysate