VE Cadherin (CDH5) (Extracell. Dom) [BV9] FITC, Mouse Monoclonal Antibody

Partner: OriGene Technologies

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Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI.