Ordering Details
Cat Number:	AM2237b
Size:	400uL
Price:	£304.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

PCNA [C-term] [1157CT1.1.3], Mouse Monoclonal Antibody

Partner: Abcepta, Inc.

Applications:	IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Isotype:	IgG1
Purification:	Mouse Monoclonal Antibody
Description:	This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Further Information:	This PCNA antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 236-261 amino acids from the C-terminal region of human PCNA.
Synonym:	Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Ascension Number:	P12004
NCBI EntrezGene:	5111
Shipping Conditions:	Ship on cold packs
Storage:	Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:	Research use only

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All lanes : Anti-PCNA Antibody (C-term) at 1:2000 dilutionLane 1: C2C12 whole cell lysateLane 2: C6 whole cell lysateLane 3: Hela whole cell lysateLane 4: L929 whole cell lysateLane 5: MCF-7 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDaBlocking/Dilution buffer: 5% NFDM/TBST.