Detection of endogenous LC-3: Whole cell lysates of untreated tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with LC3 antibody (Clone: 2G6) at 0.5 ug/ ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK 293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT Lane 8: Neuro2A Lane 9: NIH-3T3