LC3B (MAP1LC3B) (N-term) (incl. pos. control) [5F10], Mouse Monoclonal Antibody

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Endogenous LC-3 punctae detected with AM20212PU-N LC3 antibody (Clone 5F10): The majority of LC-3 was diffusely localized in unstim ulated COS-7 cells, wheres punctated signals of LC-3 increase after induction of autophagy by vinblastin stim ulation for 2 hr. Cells were fixed with paraformaldehyd followed by methanol treatment. Cells were permeabilized with 0.3% TritonX100. Endogenous LC-3 was detected with mab 5F10. Images by courtesy of I. Ciechomska and A. Tolkovsky, University of Cambridge, UK.