Whole lysate of serum starved SH-SY5Y cells was applied to SDS-PAGE and tranferred to a PVDF membrane. The Immunoblot was probed with the indicated monoclonal antibodies at 0.5 ug/ml for 1h at 15-22°C and developed with ECL (exposure time: 30 sec). Lane M: Molec ular Weight marker Lane 1: AM20205PU-N (Clone 7C6) Lane 2: AM20206PU-N (Clone 11C3)