Fluorescent image of Hela cell stained with EIF4E Antibody(Cat#AM1852b/SG101020AF).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EIF4E primary antibody (1:25, 1 h at 37?. For secondary antibody, Alexa Fluor� 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37?.Cytoplasmic actin was counterstained with Alexa Fluor� 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37?. EIF4E immunoreactivity is localized to Cytoplasm significantly.
