All lanes : Anti-Beta-actin Antibody at 1:4000-1:8000 dilutionLane 1: Hela whole cell lysateLane 2: HepG2 whole cell lysateLane 3: A431 whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.