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Anti-HIS Tag at 1:8000 dilution + 12-tag lysateLysates/proteins at 20ng per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.