Ordering Details
Cat Number:	AM1010a-ev
Size:	50uL
Price:	£117.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

HIS Tag [6AT18], Mouse Monoclonal Antibody

Partner: Abcepta, Inc.

Applications:	WB
Species Reactivity:	Human
Isotype:	Mouse IgG1
Purification:	Mouse Monoclonal Antibody
Description:	Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Further Information:	Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.
Shipping Conditions:	Ship on cold packs
Storage:	Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:	Research use only

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Anti-HIS Tag at 1:8000 dilution + 12-tag lysateLysates/proteins at 20ng per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.