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Cat Number:AM1008a-ev
Size:50uL
Price:£117.00
Quantity:
Shipping:£12.00

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MSDS

 

HA Tag [12CA5], Mouse Monoclonal Antibody

Partner: Abcepta, Inc.

Applications:WB
Species Reactivity:Human
Isotype:Mouse IgG2b
Purification:Mouse Monoclonal Antibody
Description:Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.
Further Information:KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.
Synonym:Tag from influenza hemagglutinin protein
Shipping Conditions:Ship on cold packs
Storage:Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:Research use only

 

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Anti-HA Tag Antibody at 1:8000 dilution + 12tag recombinant proteinLysates/proteins at 20ng per lane. SecondaryGoat Anti-mouse IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.

Anti-HA Tag Antibody at 1:8000 dilution + 12tag recombinant proteinLysates/proteins at 20ng per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.