<strong>Immunofluorescent Staining:</strong> BALB/c splenocytes were incubated with Rat IgM Isotype Control, Rat anti-Mouse CD22 (2D6, Rat IgG1), Rat anti-Mouse CD8 (53-6.7, Rat IgG2a), Rat anti-Mouse CD45 (LCA) (I3/2.3, Rat IgG2b), Rat anti-Mouse CD3(C363.29B, Rat IgG2c), and Rat anti-Mouse CD45RC (GL24, rat IgM). After washing, the cells were stained with PE-labeled Mouse Anti-Rat IgM. Small Lymphocytes were gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 0.02 ug/10e6 cells.
