Figure 1. Western Blot using AM08058BT-N antibody. Recombinant hamster BiP mutant HCB1- (Lane 1), F ull-length recombinant hamster BiP (Lane 2) and Total cell lysate from Ag8.653, a mouse myeloma cell line (Lane 3) were resolved by a 10% SDS-PAGE. The proteins were transferred onto a PVDF membrane and incubated with Rat anti-BiP-Biotin followed by Streptavidin-HRP. The blot was then developed using ECL Western blot detection reagents (Amersham Life Science, Arlington Heights, IL). Note: We are gratef ul to We are gratef ul to Dr. Linda Hendershot for the recombinant hamster BiP proteins and for helpf ul s uggestions.
