Phosphospecificity Whole cell extracts of Control (Lane 1) or Ins ulin stim ulated (Lane 2) MDA-MB-213 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The Immunoblot was probed with Monoclonal InsR antibody 10C3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).