beta Catenin (CTNNB1) (core) (incl. pos. control) [9G10], Mouse Monoclonal Antibody

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Detection of endogenous b-catenin: Whole cell extracts of pervanadate (VH) treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab b-cat 9G10 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). VH treatment : 15 min lane 1: A431; lane 2: SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA231; lane 7: T47D