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Cat Number:AIS0701-100ul
Size:100uL
Concentration:1mg/mL
Price:£205.00
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Shipping:£12.00

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ISG15 [3E5], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, ICC/IF, FACS
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human ISG15 (1-157aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:ISG15, a 15 kDa protein encoded by an interferon stimulated gene, and its modification system play important roles in the innate immune response, regulation of interferon signaling, pregnancy, and several cancers. ISG15 is an ubiquitin-like protein containing two ubiquitin homology domains and becomes conjugated to a variety of proteins when cells are treated with type I interferon or lipopolysaccharide.
Further Information:The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.
Synonym:Interferon-stimulated gene 15 kDa ubiquitin-like modifier, GIP2, uCRP, IFI15,ISG15,Interferon-stimulated gene, 15 kDa ubiquitin-like modifier, Interferon-stimulated gene, 15 kDa ubiquitin-like modifier IFI 15, Interferon alpha inducible protein, Interferon induced 15 kDa protein, Interferon induced 17 kDa protein, Interferon induced 17 kDa/15 kDa protein, Interferon Stimulated Gene 15, Interferon stimulated protein 15 kDa, ISG 15, ISG15 ubiquitin like modifier, ubiquitin cross reactive protein precursor.
Ascension Number:P05161
NCBI EntrezGene:NP_005092
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysateLane 2. : MCF7 cell lysate

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysateLane 2. : MCF7 cell lysate

ICC/IF analysis of ISG15 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ISG15  antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of ISG15 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ISG15 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).