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Cat Number:AHS0716-50ul
Size:50uL
Concentration:1mg/mL
Price:£123.00
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Shipping:£12.00

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HSP40/DNAJB1 [k1C7], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB
Species Reactivity:Human
Isotype:Mouse IgG2a kappa
Immunogen:Recombinant human Hsp40(1-340aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:Human Hsp40 (heat shock protein with molecular size of 40kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. Hsp40 protein contains the J-domain which controls interaction with the ATPase domain of Dnak.
Further Information:The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.
Synonym:Heat shock 40 kDa protein 1, DNAJB1, DNAJ1, HDJ1, HSPF1, HSP40 (DNAJ), Heat shock 40 kDa protein 1, Heat shock protein 40, HSP40, DnaJ protein homolog 1, HDJ-1, DnaJ homolog subfamily B member 1, Heat shock 40 kDa protein 1, radial spoke 16 homolog B (Chlamydomonas), Hsp40, Sis1, RSPH16B,
Ascension Number:P25685
NCBI EntrezGene:NM_006145
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The Cell lysates of Jurkat (50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: HSP40 Transfected 293T cell lysate

The Cell lysates of Jurkat (50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: HSP40 Transfected 293T cell lysate