Ordering Details
Cat Number:	AHR0401-100ul
Size:	100uL
Concentration:	1mg/mL
Price:	£205.00
Quantity:
Shipping:	£12.00

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MSDS

SET 7/9 [s4E5], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, ICC/IF, FACS
Species Reactivity:	Human
Isotype:	Mouse IgG2b kappa
Immunogen:	Recombinant human SET7/9 (1-366aa) purified from E. coli
Conjugation:	Unconjugated
Description:	Set7/9 is a histone methyltransferase (HMTase) that transfers methyl group to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. Set7/9, unlike most other SET proteins, is exclusively a mono-methylase.
Further Information:	The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtainoptimal results
Synonym:	SET domain-containing protein 7, SETD7, KIAA1717, KMT7, SET7, SET9, SET7/9 Histone methyltransferase, EC 2.1.1.43, Histone H3-K4 methyltransferase SETD7, H3-K4-HMTase SETD7, SET domain-containing protein 7, SET7/9, Lysine N-methyltransferase 7, Histone-lysine N-methyltransferase SETD7, SET domain-containing protein 7 FLJ21193, H3 K4 HMTase, H4 lysine-4 specific, Histone H3 K4 methyltransferase, Histone H3 lysine 4 specific methyltransferase, Histone H4-K4 methyltransferase, Histone lysine N methyltransferase H3 lysine 4 specific SET7, Histone-lysine N-methyltransferase, Lysine methyltransferase, SET 7, SET 7/9, SET 9, SET D7, SET domain containing protein 7, SET domain-containing protein 8,
Ascension Number:	Q8WTS6
NCBI EntrezGene:	NP_085151
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The Recombinant human SET7/9 protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: SET7/9 transfected 293T cell lysate

ICC/IF analysis of SET7/9 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SET7/9 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).