Ordering Details
Cat Number:	AGT0501-50ul
Size:	50uL
Concentration:	1mg/mL
Price:	£123.00
Quantity:
Shipping:	£12.00

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MSDS

GST [1E5], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, ICC/IF
Isotype:	Mouse IgG2b kappa
Immunogen:	Recombinant Glutathione S transferase (GST) purified from E. coli
Purification:	By protein-G affinity chromatography
Conjugation:	Unconjugated
Description:	Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GST proteins can be purified by immobilized glutathione affinity chromatography. Many recombinant proteins have been engineered with GST tags to facilitate the detection, isolation and purification of these proteins. Monoclonal antibody reacting specifically with GST may be useful in various immunotechnique, to identify the expression of a GST fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a GST fusion protein expressing vectors
Further Information:	The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.
Synonym:	Glutathione S-Transferase, GST, GST 26, Sj26 antigen, SjGST
Ascension Number:	N/A
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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Recombinant GST (28kDa) and GST-fusion protein (61kDa) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GST antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Arrows indicate GST and GST-fusion proteins(20ng).

ICC/IF analysis of GST in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GST antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).