Ordering Details
Cat Number:	AF6261-100ul
Size:	100uL
Concentration:	1mg/mL
Price:	£215.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

pan-AKT1/2/3, Rabbit Polyclonal Antibody

Partner: Affinity Biosciences

Applications:	WB, IHC, IF/ICC, IP, ELISA
Species Reactivity:	Human, Mouse, Rat
Isotype:	Rabbit IgG
Immunogen:	A synthesized peptide derived from human Akt, corresponding to a region within the internal amino acids.
Purification:	The antiserum was purified by peptide affinity chromatography using SulfoLink� Coupling Resin (Thermo Fisher Scientific).
Conjugation:	Unconjugated
MW:	55kDa
Shipping Conditions:	Ship on cold packs
Storage:	Store at -20 C. Stable for 12 months from date of receipt.
Usage:	Research use only

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AF6261 at 1/100 staining human brain cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.

Western blot analysis of Akt Antibody expression in Various cell/tissue lysates.

AF6261 staining MCF-7 cells by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.