Western blot analysis of MMP-8 expression in NIH/3T3 cells.The lane on the left is treated with the antigen-specific peptide.
AF5446 staining LOVO cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37C.
Western blot analysis of extracts from various samples, using MMP-8 Antibody. Lane 1: Hybridoma cells, treated with blocking peptide; Lane 2: Hybridoma cells; Lane 3: 293.