AF5248 at 1/100 staining human Liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Western blot analysis of extracts from various samples, using Actinin alpha 2/3 Antibody. Lane 1: HepG2 treated with blocking peptide; Lane 2: HepG2; Lane 3: Rat brain; Lane 4: Mouse brain.
AF5248 staining LOVO cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37C.The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37C.
