AF5161 at 1/100 staining human Pancreas cancer tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hou
Western blot analysis of extracts from various samples, using Lamin B Antibody. Lane 1: Rat brain treated with blocking peptide; Lane 2: Rat brain; Lane 3: Myeloma cells; Lane 4: Rat heart; Lane 5: HUVEC.
AF5161 staining lovo cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37C.
