Ordering Details
Cat Number:	AF3358-100ul
Size:	100uL
Concentration:	1mg/mL
Price:	£215.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

p-Histone H3 [Ser11], Rabbit Polyclonal Antibody

Partner: Affinity Biosciences

Applications:	WB, IHC, IF/ICC, ELISA
Species Reactivity:	Human, Mouse, Rat, Spodoptera frugiperda
Isotype:	Rabbit IgG
Immunogen:	A synthesized peptide derived from human Histone H3 around the phosphorylation site of Ser11.
Purification:	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Conjugation:	Unconjugated
MW:	17kDa
Shipping Conditions:	Ship on cold packs
Storage:	Store at -20 C. Stable for 12 months from date of receipt.
Usage:	Research use only

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AF3358 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.

The expression of p-CDK2 and p-Histone H3 induced by knockdown of Rictor.

AF3358 staining HepG2 by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.