AF3330 at 1/100 staining human Kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Western blot analysis of Phospho-SOX-9 (Ser181) expression in various lysates
AF3330 staining A549 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37C.The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37C.