AF3178 at 1/200 staining Human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Western blot analysis of Phospho-ATF2 (Thr69 or 51) expression in various lysates
AF3178 staining Hela by IF/ICC.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod