Ordering Details
Cat Number:	AF1023-100ul
Size:	100uL
Concentration:	1mg/mL
Price:	£215.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

Acetyl-Histone H3 [Lys18], Rabbit Polyclonal Antibody

Partner: Affinity Biosciences

Applications:	WB, IHC, IF/ICC, ELISA
Species Reactivity:	Human, Mouse, Rat, Monkey
Isotype:	Rabbit IgG
Immunogen:	A synthesized peptide derived from human Histone H3 around the acetylation site of Lys18.
Purification:	The antiserum was purified by peptide affinity chromatography using SulfoLink� Coupling Resin (Thermo Fisher Scientific).
Conjugation:	Unconjugated
MW:	15 kDa
Shipping Conditions:	Ship on cold packs
Storage:	Store at -20 C. Stable for 12 months from date of receipt.
Usage:	Research use only

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AF1023 at 1/100 staining human Brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.

Western blot analysis of extracts of various celllines,using Acetyl-Histone H3 (Lys18) Antibody.

AF1023 staining HepG2 by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.