AF1021 at 1/100 staining human Brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Acetyl-Histone H4 (Lys12) Antibody. The lane on the left is treated with the synthesized peptide
AF1021 staining Hela by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.
