AF0908 at 1/100 staining human lung carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Western blot analysis on 293 cell lysate using Phospho-Akt(Ser473) Antibody,The lane on the left is treated with the antigen-specific peptide.
AF0908 staining 293 cells by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.
