Ordering Details
Cat Number:	AF0155-200ul
Size:	200uL
Concentration:	1mg/mL
Price:	£269.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

ERK1/2, Rabbit Polyclonal Antibody

Partner: Affinity Biosciences

Applications:	WB, IHC, IF/ICC, IP, ELISA
Species Reactivity:	Human, Mouse, Rat, Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Monkey, Fish
Isotype:	Rabbit IgG
Immunogen:	A synthesized peptide derived from human ERK1/2, corresponding to a region within C-terminal amino acids.
Purification:	The antiserum was purified by peptide affinity chromatography using SulfoLink� Coupling Resin (Thermo Fisher Scientific).
Conjugation:	Unconjugated
MW:	42kDa,44kDa
Shipping Conditions:	Ship on cold packs
Storage:	Store at -20 C. Stable for 12 months from date of receipt.
Usage:	Research use only

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AF0155 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.

Western blot analysis on Hela cell lysate using ERK1/2 Antibody

AF0155 staining lovo cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37C.The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37C.